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资料内容 |
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生物活性
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Leupeptin hemisulfate is a membrane-permeable thiol protease inhibitor that inhibits Cathepsin B, Cathepsin H and Cathepsin L, and also impairs amphisome-lysosome fusion[1]. Leupeptin hemisulfate also exhibits anti-inflammatory effect[2].
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IC50 & Target
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Cathepsin[1]
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体内研究
(In Vivo)
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Leupeptin (0-36 mg/kg; intraperitoneal injection; for 4 hours; C57BL/6NCrl male mice) is well tolerated by the animals and produces a strong, dose-dependent increase in LC3b-II in both the total tissue extracts and the lysosome and autophagosome-enriched pellet fraction[2]..
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Animal Model:
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C57BL/6NCrl male mice (6-8 weeks old, 20-25 g)[2]
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Dosage:
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0 mg/kg, 9 mg/kg, 18 mg/kg, 36 mg/kg
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Administration:
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Intraperitoneal injection; for 4 hours
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Result:
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Promoted the accumulation of LC3b-II in mouse liver.
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分子量
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475.59
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Formula
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C??H??N?O? . ?/?H?SO?
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CAS 号
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103476-89-7
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Sequence Shortening
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Ac-LLR-CHO
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中文名称
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亮肽素
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SMILES
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O=C[C@@H](NC([C@@H](NC([C@@H](NC(C)=O)CC(C)C)=O)CC(C)C)=O)CCCNC(N)=N.[0.5H2SO4]
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运输条件
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Room temperature in continental US; may vary elsewhere.
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储存方式
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Powder
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- 80°C
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2 years
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-20°C
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1 year
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In solvent
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-80°C
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6 months
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-20°C
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1 month
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溶解性数据
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In Vitro:
H2O : ≥ 19 mg/mL (39.95 mM)
* "≥" means soluble, but saturation unknown.
配制储备液
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1 mM
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2.1027 mL
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10.5133 mL
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21.0265 mL
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5 mM
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0.4205 mL
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2.1027 mL
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4.2053 mL
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10 mM
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0.2103 mL
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1.0513 mL
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2.1027 mL
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*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
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参考文献
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[1]. Haspel J, et al. Characterization of macroautophagic flux in vivo using a leupeptin-based assay. Autophagy. 2011 Jun;7(6):629-42.
[2]. Aoyagi T, et al. Biological activities of leupeptins. J Antibiot (Tokyo). 1969 Nov;22(11):558-68.
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